Journal: Molecular Therapy

Article Title: CD39 Produced from Human GMSCs Regulates the Balance of Osteoclasts and Osteoblasts through the Wnt/β-Catenin Pathway in Osteoporosis

doi: 10.1016/j.ymthe.2020.04.003

Figure Lengend Snippet: GMSCs Contribute to Bone Formation after Injection into Mice (A and B) A 50-μL Matrigel plug containing 1 × 10 5 GMSCs was implanted subcutaneously in immunocompromised mice. (A) Schematic of the transplantation model. (B) Representative gross look of subcutaneous tissue and micro-CT images illustrating organoids in a transplantation model. The quantification of bone volumes in subcutaneous tissues, n = 3. (C–E) 2 × 10 6 of GMSCs were fluorescently labeled with DiR and adoptively infused into C57BL/6 mice. (C) Schematic of the GMSC tracking model. (D) Representative digital IVIS images in different groups on 3 days after injection, n = 3. (E) Representative digital IVIS images and quantification of fluorescence intensity in different time points, n = 3. (F) GMSCs and osteoblast precursors (OBPs) cocultured (GMSCs to OBPs = 1:10) in the Transwell system; then induced osteogenesis followed with alizarin red staining. Representative gross look and image under microscope were shown under different conditions and the quantification by hexadecyl pyridinium chloride monohydrate in different treatment groups n = 3. The results represent three independent experiments (mean ± SEM), ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 by Mann-Whitney tests or t test or ANOVA test.

Article Snippet: A 50-μL Matrigel plug (Corning Matrigel Growth Factor Reduced Basement Membrane Matrix, 356231) containing 1 × 10 5 GMSCs was implanted subcutaneously, and the contralateral site was injected with a Matrigel plug as a control.

Techniques: Injection, Transplantation Assay, Micro-CT, Labeling, Fluorescence, Staining, Microscopy, MANN-WHITNEY

Journal: PLoS ONE

Article Title: Multiple Functions of Nm23-H1 Are Regulated by Oxido-Reduction System

doi: 10.1371/journal.pone.0007949

Figure Lengend Snippet: (A) Comparison of Nm23-H1 expression level and invasion potential between MDA-MB-231, invasive breast cancer cell line, and MCF-7, non invasive breast cancer cell line. Upper panel shows the expression level of Nm23-H1 and α-tubulin as a control, and lower panel, invasive potential in matrigel assay. (B) Comparison of invasion potential of MCF-7 cells reducing Nm23-H1 expression level by siRNA. Upper panel shows the expression level of Nm23-H1 and α-tubulin as a control, and lower panel, invasive potential in matrigel assay. (C) Invasion potential of MDA-MB-231 cells transiently transfected with pFlag-CMV-2, pFlag-Nm23-H1 or pFlag-Nm23-H1 C109A mutant were examined. Western analysis of cell lysates using indicated antibody were employed after separation on 12% SDS-PAGE. Cells overexpressing pFlag-CMV-2, pFlag-Nm23-H1, pFlag-Nm23-H1 C109A mutant, or pFlag-Nm23-H1 H118F mutant were seeded on matrigel-pre-coated upper chamber (3×10 4 cells/150 µL) and invasion potentials were measured (left panel). The recombinant wild type Nm23-H1 or C109A mutant were treated with 5 mM H 2 O 2 or 20 mM NEM for 1 h at 37°C, and NDP kinase activity were measured (right panel).

Article Snippet: The upper chamber of transwell was coated with 50 mg Matrigel TM basement membrane matrix (BD Biosciences) for 1 h at 37°C and then placed into 24 well chambers.

Techniques: Expressing, Matrigel Assay, Transfection, Mutagenesis, Western Blot, SDS Page, Recombinant, Activity Assay